L-isoleucine is one of the eight essential amino acids in the human body. As a raw material for the synthesis of human hormones and enzymes, it is an indispensable component in various amino acid infusion and oral amino acid preparations. At present, L-isoleucine is mainly produced by fermentation. However, due to the complex metabolic mechanism of fermentation, fermentation liquor contains a variety of paraheteric acids, such as valine and alanine, and these impurities will affect the quality of the final product. Therefore, it is necessary to separate and purify the fermentation solution. The combined extraction centrifugal extractor plays a key role in this process, significantly improving the purity and recovery of L-isoleucine.

* 1. Efficient performance of combined extraction centrifugal extractor in L-isoleucine extraction **

Using D2EHPA-kerosene as extractor and hydrochloric acid as detergent, L-isoleucine can be effectively extracted from fermentation liquid by centrifugal multistage series countercurrent process. Through its efficient centrifuge separation technology and multi-stage series countercurrent design, the purification process includes 6 stages extraction and 3 stages washing, and the recovery rate of L-isoleucine is > 90%, and the purity is > 99%.

In the multistage extraction stage, the high speed rotation and strong centrifugal force of the combined extraction centrifugal extractor can effectively separate L-isoleucine from other impurities. The multistage series countercurrent operation of the equipment ensures the gradual concentration and purification of L-isoleucine at each stage, significantly improving the extraction efficiency and product purity. The use of D2EHPA-kerosene as an extractant, combined with hydrochloric acid detergent, further optimizes the extraction process, ensuring high purity and low impurity content of the final product.

** II. The innovative structure of the combined extraction centrifugal extractor responds to complex extraction requirements **

In the process of L-isoleucine extraction, the complex components and various paraheteric acids in the fermentation solution pose many challenges. The combined extraction centrifugal extractor has successfully solved these technical problems through a series of innovative structural designs. The equipment is equipped with large aspect ratio design, CIP online cleaning system and weak stirring strong separation function, so that it can effectively adapt to the complex extraction requirements of L-isoleucine fermentation solution.

The large aspect ratio design provides a larger centrifugal separation area, improves the separation efficiency, and enables a more complete separation of L-isoleucine from other impurities. CIP in-line cleaning systems ensure the cleanliness of equipment during operation, reducing downtime and maintenance costs. The weak stirring and strong separation function effectively avoids emulsification and ensures the stability and high efficiency of the separation process.

The extraction process of L-isoleucine has been optimized significantly by the application of the combined extraction centrifugal extractor, and the production efficiency and product quality have been greatly improved. Its high efficiency, automation and environmental protection characteristics make it an ideal choice in the field of amino acid extraction, providing strong support for the sustainable development of the industry. The combined extraction centrifugal extractor not only performs well in L-isoleucine extraction, but also shows wide application potential in other complex plant extraction and biological product separation.